excess nhs dpeg12 biotin Search Results


nhs-dpeg12-biotin  (Vector Laboratories)
90
Vector Laboratories nhs-dpeg12-biotin
Nhs Dpeg12 Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nhs-dpeg12-biotin/product/Vector Laboratories
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excess nhs dpeg12 biotin  (Vector Laboratories)
93
Vector Laboratories excess nhs dpeg12 biotin
Excess Nhs Dpeg12 Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/excess nhs dpeg12 biotin/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
excess nhs dpeg12 biotin - by Bioz Stars, 2026-05
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nhs-dpeg 12 -biotin  (Millipore)
90
Millipore nhs-dpeg 12 -biotin
Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.
Nhs Dpeg 12 Biotin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nhs-dpeg 12 -biotin/product/Millipore
Average 90 stars, based on 1 article reviews
nhs-dpeg 12 -biotin - by Bioz Stars, 2026-05
90/100 stars
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biotin-dpeg12-nhs  (Vector Laboratories)
90
Vector Laboratories biotin-dpeg12-nhs
Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.
Biotin Dpeg12 Nhs, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin-dpeg12-nhs/product/Vector Laboratories
Average 90 stars, based on 1 article reviews
biotin-dpeg12-nhs - by Bioz Stars, 2026-05
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100mm nhs-dpeg12-biotin (qbd10198-50mg  (Millipore)
90
Millipore 100mm nhs-dpeg12-biotin (qbd10198-50mg
Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.
100mm Nhs Dpeg12 Biotin (Qbd10198 50mg, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/100mm nhs-dpeg12-biotin (qbd10198-50mg/product/Millipore
Average 90 stars, based on 1 article reviews
100mm nhs-dpeg12-biotin (qbd10198-50mg - by Bioz Stars, 2026-05
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6 mg/ml biotin-nhs solution (nhs-dpeg ® 12-biotin  (Millipore)
90
Millipore 6 mg/ml biotin-nhs solution (nhs-dpeg ® 12-biotin
Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.
6 Mg/Ml Biotin Nhs Solution (Nhs Dpeg ® 12 Biotin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6 mg/ml biotin-nhs solution (nhs-dpeg ® 12-biotin/product/Millipore
Average 90 stars, based on 1 article reviews
6 mg/ml biotin-nhs solution (nhs-dpeg ® 12-biotin - by Bioz Stars, 2026-05
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nhs dpeg 12 biotin  (Vector Laboratories)
93
Vector Laboratories nhs dpeg 12 biotin
Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.
Nhs Dpeg 12 Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nhs dpeg 12 biotin/product/Vector Laboratories
Average 93 stars, based on 1 article reviews
nhs dpeg 12 biotin - by Bioz Stars, 2026-05
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dpeg 12 biotin acid  (Biosynth Carbosynth)
N/A
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biotin-dpeg 12-dbco  (Biosynth Carbosynth)
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biotin-dpeg 12-tfp ester  (Biosynth Carbosynth)
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fmoc-n-lys-(dpeg 12-biotin)-oh-(acid)  (Biosynth Carbosynth)
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nhs-dpeg 12 biotin  (Biosynth Carbosynth)
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Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.

Journal: Frontiers in Chemistry

Article Title: Effects of Biotin-Avidin Interactions on Hydrogel Swelling

doi: 10.3389/fchem.2020.593422

Figure Lengend Snippet: Overview of the experimental design. (A) Gelatin hydrogels were modified with NHS-dPEG 12 -biotin and loaded with avidin to investigate biotin-avidin-mediated crosslinking. (B) Gelatin polymers were biotinylated by reaction with NHS-dPEG 12 -biotin via conjugation to primary amines on gelatin. MarvinSketch was used for illustrating chemical structures, substructures and reactions, Marvin 20.15.0, 2020, ChemAxon ( http://www.chemaxon.com ). (C) Hydrogels were formed by allowing biotinylated gelatin polymers, prepared with 0.1 or 1 fold molar excesses (FME) of biotinylation reagent, to gelate overnight. Then, they were either placed directly into 17.1 or 85.5 μM avidin solutions for swelling studies in the presence of avidin or incubated overnight in 85.5 μM avidin prior to swelling studies in PBS to evaluate biotin-avidin stability.

Article Snippet: After estimating the number of amine groups present as described above, a 25 mM stock solution of NHS-dPEG 12 -biotin was prepared in DMSO (Sigma-Aldrich).

Techniques: Modification, Avidin-Biotin Assay, Conjugation Assay, Incubation

Effects of biotin concentration on swelling properties in avidin swelling buffer. (A,B) Fold changes in swelling ratios compared to day 0 time point of hydrogels biotinylated with 0, 0.1, or 1 FME NHS-dPEG 12 -biotin in (A) 17.1 μM avidin or (B) 85.5 μM avidin for 7 days. n = 3, mean ± SD. Statistical significance between different FME hydrogels determined using two-way ANOVA with Tukey's post-hoc test (0 FME vs. 0.1 FME: ** p < 0.01, *** p < 0.001; 0 FME vs. 1 FME: + p < 0.05, ++ p < 0.01; 0.1 FME vs. 1 FME: # p < 0.05). (C–E) Fold changes in swelling ratios of hydrogels with (C) 0 FME, (D) 0.1 FME, or (E) 1 FME NHS-dPEG 12 -biotin, in either 17.1 or 85.5 μM avidin solutions, over 7 days. n = 3, mean ± SD. No statistical significance resulting from different avidin concentrations, determined using two-way ANOVA with Sidak's post-hoc test. Statistical significance between time points determined using two-way ANOVA with Tukey's post-hoc test ( % indicates p < 0.05 compared to the previous day).

Journal: Frontiers in Chemistry

Article Title: Effects of Biotin-Avidin Interactions on Hydrogel Swelling

doi: 10.3389/fchem.2020.593422

Figure Lengend Snippet: Effects of biotin concentration on swelling properties in avidin swelling buffer. (A,B) Fold changes in swelling ratios compared to day 0 time point of hydrogels biotinylated with 0, 0.1, or 1 FME NHS-dPEG 12 -biotin in (A) 17.1 μM avidin or (B) 85.5 μM avidin for 7 days. n = 3, mean ± SD. Statistical significance between different FME hydrogels determined using two-way ANOVA with Tukey's post-hoc test (0 FME vs. 0.1 FME: ** p < 0.01, *** p < 0.001; 0 FME vs. 1 FME: + p < 0.05, ++ p < 0.01; 0.1 FME vs. 1 FME: # p < 0.05). (C–E) Fold changes in swelling ratios of hydrogels with (C) 0 FME, (D) 0.1 FME, or (E) 1 FME NHS-dPEG 12 -biotin, in either 17.1 or 85.5 μM avidin solutions, over 7 days. n = 3, mean ± SD. No statistical significance resulting from different avidin concentrations, determined using two-way ANOVA with Sidak's post-hoc test. Statistical significance between time points determined using two-way ANOVA with Tukey's post-hoc test ( % indicates p < 0.05 compared to the previous day).

Article Snippet: After estimating the number of amine groups present as described above, a 25 mM stock solution of NHS-dPEG 12 -biotin was prepared in DMSO (Sigma-Aldrich).

Techniques: Concentration Assay, Avidin-Biotin Assay